Verma and Pandey
of Bupivacaine Hydrochloride and Promethazine as
for 24 hours prior to each treatment and on a particular day
epidural anesthetic on Biochemical attributes of Dogs.
only one animal was used for the specific treatment. Each
animal was subjected to the following four treatments at
MATERIALS AND METHODS
an interval of 8 days.
Six apparently healthy nondescript adult dogs weighing 15
Treatment I
-
Promethazine at 4 mg/kg body weight
to 20 kg were used for the present study. The animals were
was given epidurally at the lumbo –
dewormed 10 days prior to the commencement of actual
sacral space.
experiment. Four blood samples were collected from each
Treatment II -
Simultaneous
injections
of
animal, during pre-experimental period and each sample
promethazine at 4mg/kg body weight
was analyzed for different biochemical attributes, with
and Bupivacaine (2%) at 2 mg/kg body
a view to obtain baseline values. The animals were kept
weight were given epidurally at the
under uniform diet and managemental practice during
lumbo – sacral space.
the entire experimental period. Six dogs were subjected
to successive two treatments and each of which lasted
Nine serum samples from each dog were collected at 0,
for 6 days. Eight days interval after the last collection of
3, 6, 12, 24, 48, 72, and at 96 hours post treatment for the
previous treatment was allowed before starting the next.
estimation of biochemical parameters viz; Blood Glucose,
Residual effect of the preceding treatment was ascertained
Serum Glutamic Pyruvic Transaminase (SGPT), Serum
by restoration of normal haemato-biochemical profile (to
Alkaline Phosphatase (SAP), Total Bilirubin and serum
base line values). All experimental animals were starved
creatinine. All the estimations were done by ECOM-F
photometer.BloodproteinwasestimatedbyRefractometer.
Table 1. Mean level ( ± SE) of biochemical attributes at different time Intervals in dogs given Promethazine with and without
Bupivacaine
blood creatinine
Blood Glucose
Serum Total Protein
Serum Alkaline
SGPT (IU/L)
(mg/dl)
Hrs
(mg/dl)
(gm/%)
Phosphatase (IU/L)
I
II
I
II
I
II
I
II
I
II
88.33 ±
5.77 ±
6.03 ±
30.16 ±
29.00 ±
42.67 ±
38.17 ±
0.77 ±
0.69 ±
0
82.83 ± 3.80 a
5.04 a
0.12 ab
0.08 a
2.12 ab
1.26 ab
2.08 a
1.14 a
0.08 a
0.07 a
98.00 ±
90.67 ±
5.70 ±
6.33 ±
36.17 ±
37.83 ±
48.33 ±
42.83 ±
0.81 ±
0.76 ±
3
6.42 ab
3.10 ab
0.10 ab
0.07 ab
1.28 def
0.47 c
2.36 bcd
0.91 bcd
0.09 a
0.06 ab
113.5 ±
101.50 ±
5.67 ±
6.77 ±
38.17 ±
40.50 ±
49.50 ±
45.17 ±
0.85 ±
0.80 ±
6
5.32 bcd
5.61 bc
0.11 a
0.13 cd
1.93 ef
0.89 cd
2.32 cdc
1.54 bcdc
0.09 ab
0.06 abc
120.83 ±
104.67 ±
5.73 ±
7.13 ±
42.67 ±
43.16 ±
52.83 ±
48.17 ±
0.86 ±
0.84 ±
12
6.65 cde
5.64 bc
0.12 a
0.08 de
1.23 f
1.25 d
1.97 e
1.38 e
0.09 ab
0.07 abc
126.83 ±
110.67 ±
5.87 ±
7.17 ±
34.83 ±
36.83 ±
51.67 ±
46.83 ±
0.54 ±
0.88 ±
24
5.65 de
5.54 cd
0.11 ab
0.06 e
1.19 cde
0.90 c
1.76 dc
1.28 de
0.07 ab
0.07 bc
133.00 ±
116.33 ±
6.13 ±
6.97 ±
32.50 ±
31.16 ±
50.00 ±
45.17 ±
1.01 ±
0.93 ±
48
4.95 e
5.04 cd
0.18 b
0.06 cd
1.12 bcd
1.01 b
2.24 cdc
1.4 cde
0.07 b
0.06 c
136.00 ±
125.83 ±
5.97 ±
6.77 ±
32.00 ±
29.67 ±
48.33 ±
44.00 ±
0.90 ±
0.73 ±
72
5.3 e
6.27 d
0.14 ab
0.16 cd
1.65 bc
0.33 ab
2.19 bcd
1.67 bcdc
0.06 ab
0.05 ab
109.17 ±
100.67 ±
5.83 ±
6.65 ±
29.33 ±
29.00 ±
46.5 ±
42.33 ±
0.83 ±
0.72 ±
96
4.96 bc
6.61 bc
0.13 ab
0.16 bc
1.17 ab
0.37 ab
2.16 abc
1.56 abc
0.7 a
0.05 ab
99.00 ±
88.16 ±
5.87 ±
6.07 ±
27.33 ±
26.83 ±
45.17 ±
40.67 ±
0.78 ±
0.69 ±
120
6.73 ab
3.84 ab
0.08 ab
0.16 a
1.15 a
0.65 a
2.24 ab
1.36 ab
0.09 a
0.05 a
330
Journal of Animal Research: v.5 n.2. June 2015