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IJASE - Volume 7 - Issue 2

[<<< GO BACK ][ VOLUME 7 - ISSUE 2 ]

Title: Contents Vol. 7, No. 2, December 2019
Abstract :
Title: Comparison of Candida tropicalis and Recombinant Escherichia coli BL21 (DE3) Expressed Enzyme Production for Bioconversion of Lignocellulosic Materials
Abstract :

Lignocellulose is the most abundant renewable resource naturally available with great potential for bioconversion to value-added bio-products in this case from coconut husk to reducing sugars with bio-converting enzyme(s) from Candida tropicalis and Escherichia coli BL21(DE3). This study was to colometrically analyze the xylitol bioconversion and PAGE analysis expressed natively and recombinantly. Pretreated coconut husk (PC) and carboxymethylcellulose (CMC) were used as the carbon source in minimal media to monitor the enzymatic activity, reducing sugar and enzyme production. Enzymatic hydrolysis was facilitated through fermentation of different compartments of C. tropicalis and recombinant E.coli BL21(DE3) between 0 to 96 hours. After 2 hour of induction, recombinant E. coli BL21(DE3), 0.224 g/L (IN) showed higher enzymatic activity than C. tropicalis, 0.181 g/L (EX). Bands expressed extracellularly by C. tropicalis had an estimated molecular weight of 45 kDa while recombinant E. coli BL21(DE3) was 7 kDa. Mannitol dehydrogenase had the highest similarity to the expected peptide sequence through MALDI-TOF MS analysis. Recombinant strains are developed to obtain a higher enzymatic activity compare to the native one. Future studies on optimization of production media may be done.

Title: Isatin Derived Mixed Ligand Metal Complexes as Antimicrobial, Antioxidant Agents and Catalyst for Oxidation of Styrene
Abstract :

A novel series of mixed ligand transition metal complexes of Cu(II), Zn(II), Ni(II) and Co(II) have been synthesized from the Schiff base ligand, 3-(naphthalen-1-ylimino)indolin-2-one (achieved by the condensation of isatin and naphthylamine) and 1, 10-phenonthroline. The structural features were corroborated by means of elemental analyses, molar conductance, IR, UV–Vis, 1H NMR and mass spectral studies. The data displays that these complexes have the composition of [MLX] type where X = 1, 10-phenonthroline. The UV–Vis. and magnetic susceptibility data of the complexes suggest a square–planar geometry around the central metal ion. The IR spectral data of the ligand exhibits the presence of iminic group and its complexes manifest that the imine group of the Schiff base coordinate with the metal ion and decreased value of carbonyl group indicates which coordinate to the metal. The proton NMR spectrum of the ligand shows the presence of imine group. The mass spectral data also confirms the metal complexes as [MLX] type. Molar conductance values of the complexes have been found in the range of 5 - 14 ohm−1cm2mol−1, suggesting the non-electrolytic nature of the complexes. The antimicrobial study reveals that copper and cobalt complexes are better antimicrobial agents than the standard drug streptomycin and Nystatin. The copper and cobalt complexes exhibit remarkable antioxidant potential than the other complexes. The catalytic oxidation of styrene by H2O2 was carried out in acetonitrile in the presence of mixed ligand metal complex as catalyst. The result of the catalytic study reveals that the zinc complex effectively catalyses the oxidation of styrene.

Title: A Method to Convert Regular Expression into Non- Deterministic Finite Automata
Abstract :
Non-deterministic finite automata are a part of finite automata which can accept only valid strings to process. It is needed to accept valid strings. Regular expression is used for pattern matching for strings. Regular expression cannot give the number of states for a particular automaton. That’s why, we need NFA through which we can state total number of states in any automata. There are several methods to convert non-deterministic finite automata into regular expression but there are few to convert regular expression to non-deterministic finite automata. In this paper, we proposed a method through which one can convert regular expression to non-deterministic finite automata by decomposing the input string with the help of JFLAP tool.
Title: Information Technology and Computing Programs in the Universities of West Bengal: An Analytical Case Study
Abstract :

Information and Computing is the core for development of different kind. Computing and Information Technology tools are responsible for information related activities. There are different subjects fall under the computing and IT and among these few important are include Computer Science, Computer Engineering, Computer Applications, Information Science, Information Technology, Informatics, Information Systems etc. However only few are popular among these branch and among these Computer Science, Computer Applications and Information Technology are important one. India is a large country and there are different stakeholders of various means. As far as West Bengal is concerned it is one of the important and active state having good fame in heritage, population and education. There are different educational institutes around the state and many of them offering different level of programs and different nomenclatures in IT and Computing. Conventionally in the universities of West Bengal, one university department offers a specific subject or field. But this study is undertaken to show the differences or changes of thought in this regard. Moreover, the study highlights the degrees offered by the newer and old university as well.