The wetland of the east Kolkata is spread over 12,500 hectares and comprises a large number of water. In Calcutta many poor people depend on the vegetable and fish produced here for their living. The sewage of Kolkata has got a new meaning because of a huge number of farmers and fisherman who used the city waste products as a useful resource for the traditional practice of fisheries and agriculture instead of harming the public. The objective of this study is to conduct a survey and collect databases regarding the lifestyle and disease of local people. It was an observational study done in February 2019, around the slums of East Kolkata Wetland. A schedule was prepared including an observational checklist for assessing different aspects. According to this report there are five major police stations under EKW area and this includes total 264 bheris. Maximum people depend of fish cultivation for their survival having monthly income range from 1000-10000 rupees per month. Maximum number of people are literate up to primary level and the rate is very low when it comes to higher secondary education. According to our survey maximum people said to have fever or cholera or diarrhea or other related issues. And the percentage is very high for fever. The present study found that the slum people around EKW is very much dependent on the wetland for their living and income and they are very prone to wetland related diseases.

Mangroves are inhabited by large number of fungal communities, known as manglicolous fungi. They include mostly marine fungi and small group of terrestrial fungi and can be categorized into saprophytic, parasitic, and symbiotic fungi. Fungi in mangrove environment play an important ecological role in decomposition of organic matter by production of variety of extracellular degradative enzymes such as cellulase, xylanase, pectinase, amylase, and so on. Such enzymes can be isolated from the mangrove fungi and harnessed for several biotechnological applications; mangrove fungi focused on their ecological roles as their extreme importance in nutrient cycling. Most classical plant hormones are also produced by pathogenic and symbiotic fungi.

Sophora flavescens (S. flavescens), which is used in traditional Chinese medicine, It showed potent Na+-glucose co-transporter (SGLT) interruption activity. SGLT interruption have therapeutic potential inhibitor for diabetes mellitus. S. flavescens have diverse substantial activities for anti-oxidant, anti-diabetic, anti-bacterial, anti-asthmatic, anti-pyretic, anti-ulcerative, anti-arrhythmic, anti-inflammatory, and anti-neoplastic effects. Sophora species are widely used in the therapy of many diseases such as cancer, asthma, ulcer, diabetes and it’s also used for oilments. Sophora is rich in alkaloids, flavonoids, triglycosides, isoprenylated flavonoids, isoflavonones, saponins, triterpenoid glycosides, phospholipids and polysaccharides. S.flavescens is derived compounds such as Maackiain, Variabilin, Pterocarpin, Sophoraflavone G and Formononetin etc. NIDDM is a chronic disease associated with the metabolic impairment of insulin actions, dominant to the evolution of serious complications. SGLT-2 inhibitors provide a unique way to treat the diabetes mellitus by decreasing hyperglycemia through increased glucosuria. This explosion decreases renal glucose tubular constriction in the proximal renal tubules imparting an insulin independent mechanism to curtail blood glucose. Homology modeling was performed using Schrodinger Glide 11.9 Advanced Homology Modelling to anticipate the 3D Complex of SGLT-2. In this present study, we established a novel SGLT2 inhibitor by means of docking. Docking was carried out using GLIDE software. The ligand Formononetin with the Glide score -5.074, shows the binding affinity with the amino acid (AA) residues TYR263, GLN428, GLN69, TYR87 and GLU88. These residues are acting as a conclusive pocket for the potential ligand. Hence, it has been concluded Formononetin as a potent inhibitor for SGLT2 Protein in Type 2 Diabetes.

The fuzzy logic and fuzzy numbers have been applied in many fields such as operation research, differential equations, fuzzy system reliability, control theory and management sciences etc. The fuzzy logic and fuzzy numbers are widely used in engineering applications also. In this paper we first describe Trapezoidal Fuzzy Number (TrFN) with arithmetic operations and solve a linear programming problem by Trapezoidal Fuzzy Number (TrFN) using simplex algorithm.