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Ibioinformatics - Volume 8 - Issue 2

[<<< GO BACK ][ VOLUME 8 - ISSUE 2 ]

Title: Contents
Abstract :
Title: In vitro management of Sclerotium rolfsii Sacc. – The causal agent of stem rot of sesame (Sesamum indicum L.)
Abstract :
An investigation was carried out during the tenure of March–September 2014 in search of fungal diseases of sesame. Seeds of two varieties, namely, BARI Til 2 and local variety Assima were collected from Satkhira and Jamalpur districts. Seeds were sown at the experimental field plot of Botanic garden, Department of Botany, University of Dhaka. Three types of symptoms, namely, stem rot, leaf spot, and fruit rot were recorded on sesame before flowering up to harvesting. Aspergillus flavus, Aspergillus niger, Alternaria alternata, Fusarium sp., Lasiodiplodia theobromae (Botryodiplodia theobromae), Corynespora cassiicola, Curvularia lunata, Penicillium sp., and Sclerotium rolfsii were isolated from plant parts on PDA medium following tissue planting method at temperature 25–28°C and pH 6.0. Five fungicides, viz. Homai (Carbendazim), CM-75 (Carbendazim + Mancozeb), Ridomil Gold (Mancozeb + Matalexil), Score (Difenoconazole), and Tilt 250 EC (Propiconazole) at 50, 100, and 200 ppm concentration were used to control test pathogen S. rolfsii in vitro. Tilt 250 EC found most effective and completely inhibited the radial growth of test pathogen at all the concentrations used.
Title: Phylogenetic analysis of few dehydration-responsive element-binding homologs in Poaceae
Abstract :
Nucleotide sequence of dehydration-responsive element-binding (DREB) gene from Oryza sativa was retrieved and taken for the study. BLAST was performed on the retrieved sequence and 10 nucleotide sequences were selected from the hits, which belonged to the family Poaceae. MEGA X software was used to do multiple alignments and to perform phylogenetic analysis. Concerning DREB gene phylogenetic study is carried out among the 10 plants (O. sativa, Oryza glaberrima, Oryza brachyantha, Oryza coarctata, Brachypodium distachyon, Avena sativa, Poa pratensis, Hordeum vulgare, Hordeum brevisbulatum, and Schedonorus arundinaceus).
Title: Micropropagation of Leucaena leucocephala from in vitro cultured shoot tip explants
Abstract :
Multiple shoots have been produced from shoot tip explants excised from in vivo grown plants on MS + BA medium. These shoots produced multiple roots when subcultured on a fresh MS medium containing IAA. The browning of explants was inhibited using polyvinylpyrrolidone which is supposed to absorb polyphenols as the main cause of browning of explants.
Title: Studies on prevalence of gastrointestinal parasitic infection in sheep in Kashmir Valley
Abstract :
Sheep farming is an important source of livelihood and finance elevator for landless laborers, small and marginal farmers. A study on prevalence of gastrointestinal (GIT) parasitic infestation in sheep (Kashmir Valley) in relation to month, season, and year from March 2013 to October 2016 by fecal examination was undertaken. The overall prevalence of 55.18% was observed in the present study with prevalence (%) of Haemonchus (H)/Nematodirus (Ne), Strongyloides (S), Moniezia (M), Eimeria (E), Fasciola (F), Trichotrongylus (T), and Trichuris (Tr) as 22.54, 10.84, 9.11, 9.11, 0.14, 2.56, and 0.88, respectively. The year-wise analysis revealed highest prevalence (58.87%) in 2015-16, followed by (58.35%) 2013-14 and (40.88%) 2014-15. Seasonal variation revealed highest prevalence of GIT parasites in autumn (64.03%), followed by spring (53.73%), winter (51.27%) and summer (43.82%). Month wise prevalence of GIT parasites over the years was observed highest in September (73.32%), November (64.26%), May (60.06%) and least in July (37.95%) and August (32.67%). The year, season and month wise differences were highly significant (p<0.01).
Title: Factors affecting regeneration potential of tomato (Solanum lycopersicum) – A review
Abstract :
Tomato (Solanum lycopersicum L.) is a major fruit crop of Solanaceae family. It is consumd worldwide and has been widely used as a model plant system for understanding biological processes, functional genomics, proteomics, and metabolomics. In vitro culture, a major plant biotechnology tool, exploits the totipotent nature of plant cells In recent times, genetic transformation and genome editing of plants using plant tissue and cell culture have become an important tool in both basic and applied research by insertion or deletion of target DNA. There are several factors which affect the tissue culture and regeneration of tomato. Here, we have discussed the effect of genotype; explant type, effect of age, size and orientation of explants, effect of light and temperature, nutrient media, sugar concentration, and plant growth regulators on the regeneration of tomato.