Patel et al.

and documented using gel documentation system. Each

NTSYSpc (Rohlf, 1993). A dendrogram was constructed

experiment was repeated two times with each primer and

by using the unweighted pair group method with

those primers gave reproducible bands were considered

arithmetic average (UPGMA) with the SAHN module of

for data analysis.

NTSYSpc to show a phenetic representation of genetic

relationships as revealed by the similarity coefficient

Data analysis

(Sneath and Sokal, 1973).

The RAPD and ISSR bands were scored as present (1) or

absent (0), each of which was treated as an independent

Results and Discussion

character regardless of its intensity. By comparing the

Qualitative and quantitative analysis of genomic DNA

banding patterns of genotypes for a specific primer,

genotype-specific bands were identified. Faint or unclear

The concentration of DNA was obtained between 1000

bands were not considered. The binary data generated

to 3000 ng/µl and A260/A280 was 1.84 to 2.00, as stated

were used to estimate levels of polymorphism by dividing

by Sachdev et al ., (2003). None of the DNA samples

the polymorphic bands by the total number of scored

analyzed were of poor quality.

bands. The polymorphism information content (PIC) was

calculated by the formula: PIC = 2Pi(1−Pi) (Bhat, 2002)

RAPD analysis

where, Pi is the frequency of occurrence of polymorphic

Total 80 RAPD primers were screened, of which 29

bands in different primers. Pairwise similarity matrices

primers amplified ten or more scorable bands. PCR

were generated by Jaccard’s coefficient of similarity

amplification of DNA, using these 29 primers generated

(Jaccard, 1908) by using the SIMQUAL format of

total 5118 scorable bands with 308 loci, among them

434