Molecular characterization of bacterial leaf blight resistant

Figure 3: Dendrogram showing clustering of 30 rice genotypes constructed using UPGMA based on Jacquard’s similarity

coefficient obtained from RAPD analysis.

273 loci were found polymorphic, showing 86.68%

(NIL of Lalat) recorded maximum similarity (0.75) with

polymorphism. The PIC values ranged from 0.72 (OPC

its recurrent parent Lalat. All the NILs of Tapaswini

11) to 0.94 (OPP-06) (Table-2) with an average of 0.86.

showed low values of similarity coefficient (≤ 5.0). IET

The RAPD primers produced fragments of different sizes.

19046 (NIL of Samba Masuri) and IET 18990 (NIL of

The minimum sized fragment (95 bp) was amplified by

Pusa Basmati-1) registered 0.71 and 0.63 similarity

primer OPP 01, whereas maximum sized fragment (5713

coefficient, respectively, with their respective recurrent

bp) was amplified by primer OPP 06. The highest (100%)

parents. IET 21067 (NIL of Tapaswini) showed least

polymorphism were exhibited by primers, OPA 05, OPA

similarity values with all other NILs. Similarly, IRBB

09, OPA 12 (Rao et al., 2003), OPAB 09, OPAE 03,

60 also exhibited lower values. A dendrogram (Figure-1)

OPB 17, OPH 04, OPP 03, OPP 17 and ES 19, while the

based on UPGMA analysis, produced by pooled RAPD

lowest polymorphism (40%) was observed with primer

data showed two major clusters A and B. Cluster A

ES 22. The Jaccard’s similarity coefficient values ranged

included recurrent parents IR 64, Swarna, Lalat and their

from 0.35 (between IET 20667 and IET 21067) to 0.82

respective NILs and Tapaswini and its near isogenic

(between IET 20667 and IET 20669) (Table-3). Out of

line (IET 20672). Cluster B comprised recurrent parents

three NILs of IR 64, IET 20667 showed maximum genetic

Samba Masuri, Pusa Basmati-1 and their respective

similarity (0.74) with the recurrent parent IR 64; while

NILs, NILs of Tapaswini and susceptible check cultivars.

for Swarna, all three lines exhibited moderate degree of

Cluster A was divided into two sub clusters A1 and A2.

genetic similarity ranging from 0.63 to 0.67. IET 21063

Sub-cluster A1 was further divided into A1a and A1b.

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