Patel et al.
Figure-4: Dendrogram showing clustering of 30 rice genotypes constructed using UPGMA based on
Jacquard’s similarity coefficient obtained from SSR analysis.
Solitary genotype IR 64 formed group A1a, whereas
SSR analysis
Lalat, Swarna and their NILs, Tapaswini and NILs of IR
64 were in group A1b. Group A1b showed the maximum
PCR amplification of genomic DNA of 30 rice genotypes,
genetic proximity between NILs of Lalat to its recurrent
using 20 SSR primers generated 798 scorable bands with
parent and NILs of Swarna to its recurrent parent. Groups
average of 40 bands per primer (Table 4). The size of the
A1a and A2b suggested that all the three NILs of IR 64
bands ranged from 65 bp to 1289 bp. On an average, four
were genetically different from their recurrent parent
alleles were generated per primer. The minimum sized
IR 64. Sub-cluster A2 consists of single genotype IET
fragment (65 bp) and the maximum sized fragment (1289
20672 (NIL of Swarna). Cluster B was divided into
bp) were amplified by primer RM 139. The PIC values
two sub clusters B1 and B2. Sub-cluster B1 was further
ranged from 0.07 (SR 6) to 0.86 (RM 139). The highest
divided into group B1a and B1b. NILs of Tapaswini were
PIC value of RM 139 indicated that it would be very useful
in group B1a, whereas group B1b comprised of Samba
SSR marker for diversity analysis of rice genotypes. Out
Masuri, Pusa Basmati-1 and their near isogenic lines,
of 20 microsatellite markers studied, seven gene specific
IRBB 60, GR 11, GR 3, GR 4 and Jaya. Sub-cluster B2
SSR markers (RM 13, RM 31, RM 122, RM 164, RM
consisted of susceptible variety TN 1. More diverse NILs
390, SR 11 and RM 21) were very informative as they
(to their respective parents) can be used as parents, to
readily distinguished BLB resistant near isogenic lines
bypass marker assisted selection. Since, these NILs have
from their recurrent parents and also from susceptible
three common R genes viz., xa5, xa13 and Xa21. There
check cultivars (Blair and McCouch, 1997; Rao et al.,
will be no segregation between NILs. Hence, they can be
2003; Chu et al., 2006; Davierwala et al., 2001). Genetic
used as potential parents in breeding programmes.
similarity values (Table-5) and dendrogram (Figure-2)
revealed genetic relationship among the genotypes. The
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