Effect of Epidural Administration of Promethazine ... Biochemical Attiributes of Canines

RESULTS AND DISCUSSION

protein, while in case of promethazine with Bupivacaine

significant changes in total blood protein was observed.

Blood glucose

Which may be attributed to possible hemodilution because

of temporary inter compartmental fluid shift, as suggested

The mean value of blood glucose in dogs given

by Akhare et al . (2003).

Promethazine alone rose sharply from 88.33 ± 5.04 mg/

dl at 0 hour to 136.00 ± 5.3 at 72 hr. The trend of increase

in blood glucose shows a consistent increase during 0, 3,

Serum Glutamic Pyruvic Transaminase (SGPT)

6, 12, 24 hr observations, which differed non significantly

Significant increase (P<0.01) in serum glutamic pyruvic

from each other. The peak values were observed during

transaminase (SGPT) level was observed in dogs

48 and 72 hr, which differed significantly from earlier

administered promethazine alone between 3 to 72 h

values. The peak declined significantly at 96 and 120 hrs

having maximum increased value of 42.67 ± 1.23 at 12 h

and reached normal values as at 3 and 0 hrs respectively

with control value (0 h) being 30.16 ± 2.12 IU/L.

and differed non-significantly.

Significantly higher SGPT levels were recorded during 3,

The blood glucose level after administration of

6, 12 and 24 h when compared to baseline value at 0 hr.

Promethazine with Bupivacaine ranged from 82.83 ± 3.80

Increasing trend in SGPT levels also reported by Farheen

(0 hr) to 125.83 ± 6.27 (72 hr) which was the peak value

et al (2008). The peak value recorded at 12 h. Values at

recorded. After 72 hr the blood glucose levels declined

48, 72, 96 and 120 h were on decreasing trend and were

and matched baseline value at 120 hr (88.16 ± 3.84 vs.

significantly matching to baseline value at 0 hr. The values

82.83 ± 32.80)

ranged from 29.00 ± 1.26 (0 h) to 43.16 ± 1.25 (12 h).

The observations showed significantly similar trends in

The increase in SGPT may be attributed to metabolism of

both treatments Promethazine alone or in combination

drugs in liver and possible toxicity induced by these drugs.

with Bupivacaine. The increased blood glucose levels

During the process of elimination they may have caused

may be attributed to the increased level of adrenocorticoid

destruction of liver cells and increased cell membrane

hormones (Lee Femine et al ., 1957) as a result of stress

permeability resulting into liver edema, dilatation of liver

and suppression of insulin release (Feldberg and Symond,

sinusoids and degeneration of liver cells (Nityanand et al .,

1980).

1982) leading to SGPT level in blood.

Serum total protein

Serum Alkaline Phosphatase (SAP)

DogsgivenPromethazinealonedidnotshowanysignificant

In dogs administered Promethazine alone Serum alkaline

change in serum total protein level during the various hrs

phosphatase (SAP) showed increasing trend at 3, 6, 12,

of observations. Non significant differences were observed

24, 48 and 72 h, having reached peak value at 12 hrs and

between values at different hrs of observations. However,

then declining. Values at 3, 6, 12, 24, 48 and 72 h differed

values at 6 and 12 hrs post treatment were significantly

significantly from control value (0 hrs). However values

lower than value of total blood protein at 48 hrs which is

at 96 and 120 h matched control value and differed non

the peak value recorded.

significantly.

The administration of Bupivacaine along with

The SAP values ranged from 38.17 ± 1.14 (0 h) to 48.17 ±

prometahazine resulted in increase in total serum protein

1.38 (12 h). The peak value was recorded on 12 hr which

during different time interval. Peak value was recorded

declined during 24, 48, 72, 96 and 120 h. Values at 120

during 24 h observation, which was significantly higher

h was statistically significant compared to baseline value

than 0, 3,6,12.48,72,96 and 120 hrs. Values at 120 h were

at 0 h (40.67 ± 1.36 vs. 38.17 ± 1.14). Increased activity

similar to serum total protein value at baseline value (0 h).

of alkaline phosphatase may be attributed to changes in

The trends revealed that in case of promethazine alone

production and excretion of bile (Kelly, 1979).

non significant changes were observed in total blood

Journal of Animal Research: v.5 n.2. June 2015

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