A cross- sectional study was conducted in and around Bahir Dar, from November, 2016 to April, 2017 to estimate the prevalence of major ixodidea ticks on bovine and to identify ticks on the species level. Study animals were selected randomly. Out of the total of 384 cattle examined, 157(40.9 %) were found to be infested. About 822 adult ticks were collected both sides of animal body parts, then preserved with 70% alcohol within universal bottle and were identified to species level by using stereo-microscope. From the total ticks collected, four genera and species namely; Amblyomma varigatum, Boophilus decoloratus, Rhipicephalus evertsis and Hyalomma marginatu were identified and account for 45.3, 22.9, 15.8 and 17.03%, respectively. From different variables (sex, age, breed and body condition), body condition and predilection sites were statistically significant with tick infestation (p< 0.05). The prevalence of tick infestation was found highest in poor body condition animals (16.9%) while in medium and good body condition, it was found (14.58%) and (9.36%), respectively. It has also been evident that the favorable predilection sites of A.varigatum ticks were preferred scrotum/udder and pernial region. B.decoloratus preferred dewlap, scrotum/udder and Rh.evertis had a strong affinity on anus, dewlap and tail tip. H.marginatum the perineum region and dewlaps were its hiding sites. From this study we can make a conclusion that the prevalent ticks could also be responsible for transmission of tick borne diseases in addition to their physical damage.

The study was conducted to investigate the effects of different levels of cholesterol loaded cyclodextrin (CLC) addition on cooled and frozen-thawed spermatozoa of Marwari stallion. A total of 48 ejaculates were collected from six adult Marwari stallions (8 ejaculates from each stallion) aged between 4 to 7 years. Immediately after collection semen sample was macroscopically evaluated and filtered into a warm, graduated measuring bottle to get gel free semen. The level of cholesterol (C) and phospholipid (P) in fresh spermatozoa were measured using ELISA. The semen sample was divided in to five equal aliquots (T0, T1, T2, T3 and T4). Primary extender containing different concentrations of CLC was added to each aliquot (0, 1, 1.5, 2 and 3 mg/ml CLC in T0, T1, T2, T3 and T4, respectively). All the aliquots were incubated for 15 minutes at 37°C for incorporation of CLC in sperm plasma membrane and then cryopreserved. Level of C and P in spermatozoa was also evaluated at pre-freeze and post-thaw stages. The mean C, P and C: P ratio in fresh sperm was 15.36±0.47 μg/100 × 106 sperm cells, 46.21±1.27 μg/100 × 106 sperm cells and 0.33±0.071, respectively. The mean C content and C: P ratio were significantly higher (P<0.05) in group T3 as compared to other groups at both pre-freeze and post-thaw stages. It was concluded that addition of CLC may be helpful in increasing cryopservability of stallion spermatozoa.

Gaddi is the predominant Indian goat breed also known as “White Himalayan goat”, constituting 60-65% of total goats in the state of Himachal Pradesh. The polymorphism of prolactin receptor (PRLR) gene was found to have relationship with prolificacy in goats. In present study, polymorphism of intron 2 region of PRLR gene was investigated in Gaddi goats (n = 89) using PCR-SSCP and DNA sequencing approach. PCR-SSCP assay of 176 bp amplicon of intron 2 region of PRLR gene revealed polymorphism with three types of genotypes viz., AA, AB and BB with genotypic frequencies as 0.31, 0.55 and 0.14, respectively. The allelic frequency of alleles A and B were 0.59 and 0.41, respectively in all the screened goat population. Genetic diversity analysis revealed the value of Ne, Hobs, Hexp and PIC were 1.96, 0.52, 0.49 and 0.37, respectively. The Ne and Hobs values also indicated that sufficient genetic variation exists at the studied locus. FIS estimate was observed as -0.15 indicating heterozygous excess at studied locus. DNA sequencing of amplified product revealed one nucleotide mutation (T92C) in intron 2 region of PRLR gene. The mean litter size in AA, AB and AB genotypes were 1.27±0.12, 1.41±0.09 and 1.84±0.26, respectively. No significant (P>0.05) associations of PRLR genotypes with litter size were observed. Effect of season and parity were also found to be non-significant (P>0.05) on litter size. Consequently, the study on additional data based on more number of animals in diversified flock should be carried out for future association studies.

Secreted phosphoprotein 1 (SPP1) is popularly known as osteopontin (OPN), which plays an important role in initiation and maintenance of pregnancy, as well as in the development of the fetus and milk production. In the present study, investigation of G>T polymorphism in exon 7 region of SPP1 gene was undertaken in 147 Sahiwal and Hariana cattle maintained at Livestock Farm Complex (LFC), DUVASU, Mathura using HpyCH4IV/PCR-RFLP assay. Amplification of SPP1 exon 7 region revealed 204 bp product and HpyCH4IV restriction digestion screening showed monomorphic pattern. Only one type of genotype, namely, TT (204 bp) was observed in population. The frequency of TT genotypes was 100% in all screened samples with T allele (1.0). The results revealed that SPP1 T allele seems to be fixed in screened cattle population. Consequently, we could not perform the association study of this substitution with milk production traits.

M-mode echocardiography is a non- invasive tool for the quantitative study of heart. Rajapalayam is a guard dog and Chippiparai is a sight hound breed which both originated from the southern districts of Tamil Nadu, India. Diagnosis of cardiac diseases in our indigenous breeds has been challenging due to less or no work done regarding cardiac anatomy and physiology in native breeds. Hence, this study was undertaken to establish basic reference data in these indigenous dog breeds which will help in diagnosing cardiac diseases. Various normal cardiac parameters were documented between young and adult age groups. The results indicated that a high significant difference (P<0.01) was observed in LVDd and LVDs and only a significant difference (P<0.05) in AO and RVD values between the age groups in Rajapalayam dogs,whereas, in Chippparai dogs, a significant difference was observed in CO (P<0.05) and in LVPWs, IVSd, IVSd (P<0.0) between the young and adult age groups.

The present study was conducted on buffalo foetuses (n=19) ranging from 7.4 (62 days) to 108 cm (317 days) curved crown rump length (CVRL) to study the distribution of phosphatases and oxidoreductases in thyroid gland during prenatal development. A progressive increase in phosphatase activity from 12.50 cm CVRL (85 days) to 41 cm CVRL (166 days) was observed around the blood vessels in the developing thyroid. Mild activity of Succinate dehydrogenase (SDH) was observed at 13.50 cm CVRL (89 days) whereas Lactate Dehydrogenase (LDH) activity was absent at this stage. SDH activity was correlated with mitochondrial localization in developing thyroid. Weak to moderate LDH activity was observed at 56 cm CVRL (200 days) suggesting the presence of glycolytic pathway in developing thyroid. Mild to moderate Reduced Nicotinamide Adenine Dinucleotide diaphorase (NADH-diaphorase) and Reduced Nicotinamide Adenine Dinucleotide Phosphate Diaphorase (NADPH-diaphorase) was noticed in the follicular cells and interfollicular spaces of the thyroid gland at 12.50 cm CVRL (85 days) which progressively increased with gestational age and became intense at 56 cm CVRL (200 days) indicating increase in metabolic activity

Nigella sativa, Allium sativum and Trigonella foenum-graecum are common dietary spices also traditionally used in the treatment of various diseases including diabetes mellitus. Clinical research has confirmed the efficacy of several spices extract in the modulation of oxidative stress associated with diabetes mellitus (DM). The therapeutic activity of each individual spice is well documented, but their effect when combined is unknown. Polyherbalism is of current interest because polyherbal formulations enhance therapeutic action and reduce the concentrations of single herbs, thereby reducing adverse events. This study evaluated the hypoglycaemic and anti oxidative activity of aqueous extract of black cumin, garlic, fenugreek individual and its combination at different doses in STZ-NT-induced diabetic rats. Diabetic rats were treated with aqueous extract for 30 days. These extract significantly (p<0.05) lowered the elevated fasting blood glucose, oxidative parameters but no effect seen in haematological indices. This oxidative stress was related to a decreased superoxide dismutase activity in diabetic rats. We suggested that black cumin, garlic, fenugreek and its combination could be used as antidiabetic complement in case of Type II diabetes mellitus.

Adiponectin (ADIPOQ) gene regulates the glucose metabolism, insulin sensitivity, fatty acid oxidation, immunity and reproduction. In the present investigation, PCR-RFLP method was conducted to identify the genetic polymorphism in intron 2 region of adiponectin (ADIPOQ) gene in Indian Sahiwal cattle. PCR of intron 2 of ADIPOQ gene fragment produced amplicon of 961 bp which was subsequently digested with RsaI restriction enzyme. The RsaI/PCR-RFLP assay revealed monomorphic pattern only, TT genotype (wild type homozygote) in Sahiwal cattle population which was confirmed by sequencing. The obtained sequences of ADIPOQ after aligning revealed absence of RsaI recognition site GTAC and consequently, the association study with economic traits could not be performed.

Eight clinical cases of dogs having femur fracture, were divided into two groups, A and B with four dogs (n=4) in each group. Group A animals were treated with intramedullary pinning (IMP) alone and group B were treated using IMP alongwith demineralized bone matrix (DBM) implantation at the fracture site. The efficacy of healing was evaluated on the basis of clinical evaluation, haemato-biochemical and radiographic parameters on the day of admission (day 0), followed by 7th, 15th, 30th, 45th and 60th post-operative days. The weight bearing was observed to be better in dogs of group A. Swelling was completely absent after 15th post-operative day in dogs of both the groups. The mean values of Hb, PCV, TLC and DLC showed a non-significant variation on subsequent post-operative days in both the groups. There was a significant increase in serum calcium up to 30th post-operative day and thereafter, followed a decreasing trend. The serum alkaline phosphatase values showed non-significant variation in group A while in group B animals significant increase observed on 15th, 30th and 45th post-operative day. Radiographically, the dogs of group B showed better radiographic union of fracture evidenced by early disappearance of fracture line than those of group A.

Enterotoxigenic Staphylococcus aureus can be isolated from various sources and are responsible for food poisoning in addition to many suppurative infections. In the present study, from 82 physically normal milk samples from cattle subclinical mastitis 33(40.24%) were isolated to S. aureus. Out of these, 22 (66.66%) S. aureus isolates had either one or more enterotoxin genes. Both seg and sei enterotoxin genes were carried by 19 (57.5%) S.aureus isolates while only three (9.09%) isolates were positive for seh gene. This study showed the evidence of presence of enterotoxin genes seg, seh, and sei in S. aureus isolates from subclinical mastitis. The presence of these enterotoxigenic S. aureus strains in milk indicates potential public health concern.

The storage stability study was conducted for precooked breast fillets T1 (without post-mortem ageing, T2 (PM ageing for 4 h at room temp) and T3 (PM ageing for 24 h at refrigeration temp). Nutritional profiles were evaluated on 0 day and 15th day of aerobic storage. The samples were also evaluated for physico-chemical, microbiological and sensory parameters at 5 days interval up to 20 days. Moisture content decreased significantly (P≤0.05) from 0 day to 20th day of storage. The overall protein content was significantly lower at 20 day of storage as compared to fresh sample. Fat content showed slight decrease in T1, T2 and T3 during storage period. pH values were increased with the increase of storage intervals but after 10th day onwards the values showed decreasing trends. T3 showed least Warner Bratzler shear force value (W-BSFV) but values were decreased non-significantly. Overall thiobarbituric acid reactive substances (TBARS) values were increased with the increase of storage time, and accordingly amongst the different treatments, overall TBARS values in breast fillets were also differed significantly. Peroxide value (PV) showed significantly increasing trends for T1, T2 and T3 with advancement of storage days. Highest overall free fatty acids (FFA) content was observed for T3. T2 and T3 showed significantly higher overall titrable acidity than T1. Titrable acidity range was between 18 to 20%. Changes in microbiological quality were observed with the increase of storage days. However, these only the values of standard plate count (SPC) were much lower than the permissible limits of FSSAI for meat products. However, overall acceptability scores of breast fillets decreased significantly up to 20th day of storage period. The breast fillets of T3 sample had significantly higher overall acceptability score.

A total 500 cows were randomly selected from college livestock farm, Kuthuliya and different villages in and around Rewa (M.P.). After recording history all the animals were subjected to gynaeco-clinical examination, Whiteside test and endometrial cytology by cytobrush technique. On the basis of above tests performed all the animals were selected for the study. They were divided into three groups as follows: Normal (n=280), Clinical endometritis (n=80) and Subclinical endometritis (n=140). All the animals were subjected to aseptic collection of uterine fluid by low volume lavage technique. Uterine fluid samples obtained were used for microbial assay and antibiotic sensitivity tests. Among the bacterial isolates Staphylococcus species (36.31%) was highly prevalent. The antibiotic sensitivity of isolates was found to be maximum for ceftriaxone and sulbactum combination 91.67 per cent followed by levofloxacin 89.07, ciprofloxacin 79.69, ceftriaxone 73.43, enrofloxacin 61.45 and gentamicin 56.78 per cent, respectively. It was concluded that Staphylococcus species was highly prevalent bacteria isolated and a combination of ceftriaxone and sulbactum was found to be highly sensitive.

The study was conducted in Aizawl and Kolasib district of Mizoram, India in 375 numbers of randomly selected pig farms covering 1126 numbers of pigs. The base line survey was conducted using a structured questionnaire. The pigs under study were distributed in different age groups as < 1 months, 1-3 months, 4-6 months, 7-9 months and 10-12 months and above 1 year. The calendar year under study was divided in to four seasons viz. summer, monsoon, retreating monsoon and winter. Different breeds were classified as Yorkshire, Hampshire, Cross breed and Zovawk (indigenous).The disease conditions were observed and diagnosed on the basis of history, clinical signs, clinical, haemato-biochemical and laboratory examination like radiography and bacteriological examination and antibiotic sensitivity test as and when required. After evaluation of the lameness, the animals were treated accordingly and outcomes were evaluated. The rate of incidence of lameness was found to be 7.46 percent with a sex wise incidence of 8.67 percent in males and 5.96 percent in females. Incidence was highest in crossbreed pigs (7.57%) and in the age group of 1-3 months (12.37%). Leg wise affections were highest in foreleg (48.81%) followed by hind leg (38.10%) and both legs (13.10%). Foreleg affections were highest (54.55%) in the age group of 7-9 months and lowest (42.86%) in pigs ˃12 months. Hind leg affections (57.14%) was highest in ˃12 months and lowest (30. 34%) in the age group of 1-3 months. Sixty five percent wound cases were bacteriologically positive with 53.00 percent infected wounds of polymicrobial nature.

The study is undertaken in five organised dairy farms located in Chennai, Trichy and Karur districts of Tamil Nadu to assess the incidence of clinical and sub clinical mastitis and predisposing factors such as Herd, Breed, Parity, stage of lactation and the efficiency of two screening tests for the diagnosis of sub clinical mastitis (SCM) in the dairy farms. A total of 159 cows with 636 quarters samples screened for clinical mastitis signs and SCM by modified California mastitis test (mCMT) and somatic cell count (SCC).The results showed that 9.4 per cent of animals had clinical mastitis, 66.7 per cent had SCM by mCMT and 77.4 per cent by SCC. The quarter wise incidence of SCM was 47.6 per cent as screened by mCMT and 51.7 per cent by SCC. There are no significant differences observed in the incidence of clinical mastitis and SCM in farm and breed wise. Parity (p<0.01), Stage of lactation by SCC method (p<0.05), Quarter-wise (p<0.05) incidence have significant differences of occurrence. SCM as screened by mCMT and SCC were compared for agreement by Cohen’s Kappa statistics and the per cent agreement for the two methods of screening SCM was 54.7 per cent with a P-value of 0.000. The study conclude that the higher SCM incidence in organised dairy farms as 66.7 per cent by mCMT and 77.4 per cent by SCC and necessitate the need of effective mastitis control program to be implemented at the farm level.

Immune-mediated hemolytic anemia (IMHA) is the most common autoimmune disease in dogs. This study was conducted to study the incidence and clinicopathological changes of secondary IMHA, which is having any secondary underlying triggering causes. The anaemic dogs brought with clinical signs such as pale or icteric mucous membranes were screened for IMHA by saline agglutination and spherocyte count and confirmed by flow cytometry. The positive cases were further subjected to haematology, biochemistry, coagulation profile, MAT and polymerase chain reaction (PCR) for the diagnosis of underlying secondary causes like Babesia spp, Ehrlichia canis and Leptospira spp. (secondary IMHA). Thirty two cases were positive for IMHA, out of which thirteen cases were primary IMHA (17.3 %) and remaining nineteen cases were secondary IMHA (82.7 %) due to underlying causes such as Babesia gibsoni (13), Ehrlichia canis (3) and Leptospira spp. (3) respectively. Highest incidence was observed in Labrador dogs with age group of 2-8 years in male dogs. The most common clinicopathological findings were anaemia with reduced Hb, PCV and RBC, leucocytosis, neutrophilia, monocytosis, thrombocytopenia, elevated PT, BUN, Creatinine, ALT, ALP, hypoalbuminemia and hyperbilirubinaemia.

The tissues from small intestine containing duodenum were collected from six young goats and processed for scanning and transmission electron microscopic studies. The villi of the duodenum varied in shape and size. The duodenal mucosal surface showed leaf shaped villi along with scanty population of goblet cells on the villi surface. The mucosal surface was having very faint corrugations and dense mat of microvilli. The basal surface of the villi, the number of goblet cells was more. The crypts openings were evident at some places. The transmission electron microscopic studies revealed the presence of different cell population which included columnar or absorptive cells and goblet cells in surface epithelium of the duodenum. The columnar cells were having many uniform regularly spaced microvilli. The goblet cells were very few in the villus epithelium and were interspersed in between the columnar cells and their cytoplasm was distended with mucus granules. The crypt region or glandular epithelium was consisted of polymorphic cell population which included the Paneth cells, enterochromaffin cells and goblet cells.

The present study was carried out to assess food and foraging niches of carnivorous bird species in mixed fruit orchard selecting two transects I and II at Punjab Agricultural University campus from March 2015 to February 2016. There were observed 12 and 11 carnivorous bird species out of total 52 and 38 bird species recorded in transect I and II respectively. Habitat features (hedgerows, un-cemented water channel), irrigation schedules and fruit developmental stages seemed to determine the bird composition, especially carnivorous bird species in mixed fruit orchard.

The present study was performed to evaluate haematological and biochemical parameters with response to milk production of dairy cattle at low temperature. Ambient temperature and relative humidity were recorded and the temperature-humidity index (THI) was calculated as indicator of thermal comfort zone for cattle. Lactating crossbred dairy cattle were selected for this study. A significant increase in RBC, Hb, and PCVWBC was recorded in winter. Glucose and aspartate aminotransferase (AST), albumin level showed a significant increase in winter. Milk production level was decrease due to cold stress. These results provided an insight into the haematological and biochemical responses of Jersey crossbred cows to different environmental conditions. Hence, this study will be helpful for the better dairy cattle management in winter seasons for higher production at the cold arid high altitude region

Oxidative stress is common when the cellular antioxidant capacity is overwhelmed by the reactive oxygen species generated by the metabolism of pesticides which either inhibit or modulate the expression of antioxidant enzymes. The present study was made with objective to evaluate the ameliorating potential of Panax ginseng on oxidative stress following subacute exposure to Acetamiprid in rats. Twenty four adult male Wistar rats were divided in four groups comprising six each. Group I served as control and was administered with groundnut oil (1ml/100gm), group II was administered with Acetamiprid (52.5 mg/kg body weight), group III served as Panax ginseng (50 mg/kg body weight) control and in group IV possible ameliorative effect of Panax ginseng was examined against Acetamiprid. Vehicle, Acetamiprid and Panax ginseng were administered daily to the rats orally by gavage for 28 days and animals were sacrificed at the end. Liver, kidney, spleen and brain were processed for determination of oxidative stress related parameters viz: Lipid peroxidation (LPO), reduced glutathione (GSH), Superoxide dismutase (SOD), catalase and glutathione reductase (GR) levels. Acetamiprid produced toxicity which was evident in the form of enhanced lipid peroxidation and decrease in activities of GSH, SOD, catalase and glutathione reductase levels in all the organs examined. Panax ginseng was significantly effective in restoration of these parameters towards normal. Thus, it was concluded that Panax ginseng has ameliorating potential on oxidative stress following subacute exposure to acetamiprid in rats

An experiment was conducted to evaluate the effect of supplementation of rumen protected methionine (RPM) and rumen protected lysine (RPL) individually and in combination on nutrient utilization and growth performance in twenty Sahiwal female calves. Sahiwal female calves having age groups of 7 to 12 months and between 80 to 111 kg body weights were randomly allotted into four groups of five animals in each, viz., T1 (basal diet), T2 (basal diet with RPM 5 g/d), T3 (basal diet with RPL 15 g/d) and T4 (basal diet with RPM 5 g plus RPL 15 g/d) their body weight for 90 days. The results revealed that intake of DM and CP were similar amongst all four groups. Digestibility of nutrients was also similar amongst the groups while average daily gain was found to be better in supplemented groups.

Subclinical endometritis (SCE) is the inflammation of endometrium without systemic illness, hence remains mostly undiagnosed and untreated. The early diagnosis necessitates the evaluation of important risk factors. The objective of this study was to identify the risk factors of SCE and their effects on reproductive performance of Nili-Ravi buffalo. 100 buffaloes approaching parturition were selected. During calving, various risk factors viz., type of calving, peri-parturient disorders, sex and birth weight of calf, gestation period and season of calving were recorded. Buffaloes were subjected to endometrial cytology using cytobrush method on 45 day postpartum (DPP) and divided into 2 groups viz., buffaloes ‘with SCE’ (>5% PMN; n=38) and ‘without SCE’ (≤5% PMN; n=62). Buffaloes in estrus were artificially inseminated and fertility parameters were recorded. The occurrence of SCE was significantly affected by calving assistance (OR=11.74; P<0.001), peri-parturient disorders (OR=6.87; P<0.05) and gestation period (OR=1.16; P<0.05). Sex, birth weight of calf and season of calving were not associated with SCE. The service period of buffaloes did not vary between two groups. The median days open was significantly (P<0.05) higher in buffaloes with as compared to buffaloes without SCE (141 vs. 117 d). Buffaloes with SCE had significantly (P<0.05) lower first service conception (21.1 vs. 43.5%) and took more (P<0.05) mean number of services per conception (2.71 vs. 1.62) compared to buffaloes without the SCE. It may be concluded that risk factors around the time of calving control the onset of SCE which in turn has negative impact on reproductive performance of Nili-Ravi buffaloes.

The objective of this study was to characterize the vaginal bacterial flora and subsequent conception rates after AI in buffaloes subjected to 3 different estrous induction regimes-the use of CIDR and use of two intravaginal sponges (Polyurethane sponges with micronized progesterone and Polyurethane sponges with micronized progesterone with Carboxy methyl cellulose). The estrus induction was 100% in Group I and II followed by 91.67 % in group III. The pregnancy rates were Group I, II and III were 50.00, 66.67 and 54.55% respectively. All the vaginal swabs in all three groups yielded growth of bacteria and the predominance of mixed isolates over single isolates 81.94% vs 18.06% was indicative of dominance of mixed culture over single isolates. E. coli, Staphylococcus, Proteus and Klebsiela spp. were the commonest isolates obtained prior to insertion and after removal of implants in postpartum anestrus buffaloes. The gram positive bacteria were Staphylococcus, Streptococcus and Bacillus spp. while, gram negative bacteria were E. coli, Proteus and Klebsiella spp. These organisms could be considered as a part of the normal bacterial flora of the buffalo.

Foot-and-mouth disease (FMD) is an acute highly contagious viral disease of cloven-hoofed animals which has been discovered more than 100 years ago. The causative organism is a RNA virus belongs to the Aphthovirus genus, Picornaviridae family. The disease is endemic in India and every year the economic loss due to direct and indirect cause is more than USD 4 billion annually. The Andaman & Nicobar Islands also suffered due to the outbreak of FMD in the year 2005 which was due to serotype O. However, in the present study the reemergence of FMD virus almost after a gap of 13 years has been reported from the South Andaman district of the A & N Islands. The outbreak was confirmed as serotype O and is mainly affected more than 800 cattle, goat and buffalo population. The reemergence of the FMD virus in the islands has posed a serious concern to the livestock of this island which is now being considered as almost free from many of the dreaded disease. Based on the epidemiological investigation it could be concluded that the virus might have entered through the transport of straws which is being used as packing materials for vegetable and fruits from mainland.

The present study was conducted to extract ACE-I inhibitory peptides from duck egg using proteases from different sources viz. plant (papain and ficin), animal (trypsin) bacterial (Alcalase). Initially, the whole duck egg liquid was defatted with multiple washing with ethanol. The partially denatured whole duck egg proteins were subjected to hydrolysis using preoptimized conditions (enzyme substrate ratio, pH, temperature, incubation time) w.r.t. each enzyme. Four treatments viz duck egg hydrolysed with alcalase (DEA), duck egg hydrolysed with ficin (DEF), duck egg hydrolysed with papain (DEP) and duck egg hydrolysed with trypsin (DET) were prepared. The collected respective hydrolysates were fractionated using ultrafiltration to obtain different fractions on the basis of molecular weight (kilo dalton) viz. whole: DEPH, >10 kDa: DEPH-1, 5-10 kDa: DEPH-2, 1-5 kDa: DEPH-3 and <1 kDa: DEPH-4. The whole hydrolysates and their fractions were evaluated for their ACE-I inhibitory activity under in-vitro condition. Amongst all the hydrolysates and their fractions, the ACE-I inhibitory activity of DEPH were significantly (p<0.05) higher than their respective fractions and DEPH of DEP exhibited the highest activity. However, all the fractions displayed varied (p<0.05) ACE-I inhibitory activity with each other. Results suggested that the duck egg protein hydrolysates and their fractions have strong antihypertensive activity which can be exploited to develop nutraceuticals or functional foods.

The objectives of this study were to investigate growth patterns of Sirohi goat applying the Brody function and estimated the parameters of growth curve including genetic evaluation of growth curve traits. The data used in this study, collected in All India Co-ordinated Research Project (AICRP) on goat improvement, Livestock Research Station, Vallabhnagar, Udaipur, India from year 2009 to 2017 and were included 340 male and 715 female kids body weight measured at birth to 12th month of age in every three months interval. Least-squares means for growth curve parameters of A (Asymptotic weight), B (folding point of growth) and K (maturity rate) were 26.18±3.11 (kg), 0.88±0.00 (kg) and 0.16±0.00 (days), respectively. Fixed effects (cluster, year and season of birth, sex, and type of birth) were significantly influenced the parameters of growth curve. The heritability estimates ranged from 0.02±0.01 (“K”) to 0.40±0.05 (“A”). The genetic correlation between A-K and B-K was negative, which might be due to the compensatory growth effect.

Present study was planned to conduct the prevalence of poly-bezoars and its effect on free range axis deer (Axis axis) in around Jabalpur, India. A retrospective study was conducted in and around Jabalpur, India for February to June 2017 to observe the prevalence of poly-bezoars in wild herbivores health. During the study period 19 post-mortem examination was conducted and in 4 cases (21.05%) showed poly-bezoar. The quantity of the poly-bezoars collected from the rumen and reticulum was ranging from 0.75 to 3.5 kg. The study identifies types and estimates the prevalence of foreign bodies in the rumen and reticulum of Axis deer. Awareness may be created on careless disposal of plastic bags and as well as the periodical cleaning of these wastes in the forest/grazing areas.