The proposed work was conducted in the Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, N.D.V.S.U., Jabalpur (M.P.).

Fresh mature leaves of of Tamarindus indica were procured from, three different places of Jabalpur viz. Jawaharlal Nehru Krishi Viswa Vidyalaya (J.N.K.V.V), Tropical Forest Research (T.F.R.I.), State Forest Research Institute (S.F.R.I), Jabalpur. Samples were immediately brought to laboratory and were used within 24 hrs and finally processed for isolation of endophytic bacteria.

The sterilization of leaves and isolation of endophytic bacteria from the leaves was done according to Mahajan et al. (2014), with some modifications. The leaves were excised with autoclaved scalpel and forceps in laminar air flow.

To confirm that the surface of leaves were effectively sterilized, 1 ml of the sterile distilled water that was used in final rinse of surface sterilization procedures was placed on to nutrient agar media and incubated at 37° C for 24 hrs. Then bacterial growths were observed.

King’s B (KB) media (HiMedia), Mueller Hinton media (HiMedia), Blood agar media (HiMedia) and BHI broth (HiMedia) were prepared by adding agar into the distilled water. Hot plate was used for the proper mixing of media and autoclaved at 121° C for 15-20 minutes at 15 lbs.

The media were poured into different autoclaved small petri plates and leaves of the plant were embedded in small petri plates. These plates were then incubated at 37° C for 24 hrs. Characterization of the bacteria was done according to its morphology and by Gram’s staining. After that a single colony was transferred into BHI broth and incubated at 37° C for 24 hours.

Form, elevation, margin, surface, opacity and chromogenesis of isolated endophytic bacterial colonies were noted.

Growth characteristics of endophytic bacteria isolated from Tamarindus indica on King’s B media showed that 66.67 per cent of isolates were irregular in shape while 33.33 per cent were circular in shape, 73.33 percent showed flat elevation on petri plate while 26.67 per cent were of raised elevation, margin of 73.33 per cent colonies were undulated while 26.67 per cent showed entire, the surface of the growth was smooth for the undulated colonies and 86.67 per cent growth were opaque and white in colour.

For purification of endophytic bacteria, sub culturing was mainly done by streaking a loop full of BHI broth on the fresh pre solidified blood agar plates and then incubated at 37° C for 24 hrs. After incubation the colony was transferred into BHI broth and then incubated at 37° C for 24 hrs and purity was checked by Gram’s staining and stored for further work.

Growth characteristic of isolated endophytic bacteria from Tamarindus indica in BHI broth showed characteristics as 33.33 per cent isolates with turbidity and 80 per cent isolates with pellicle formation. Sediment formation was seen in 26.67 per cent isolates and 53.33 per cent isolate showed ring formation.

Colonies of endophytic bacteria grown on King’s B agar were transferred to 5 per cent sheep blood agar plates and incubated at 37° C for 24 hrs. All the isolates from Tamarindus indica were non-haemolytic in nature.

The smear was prepared and fixed. Gently flooded the smear with Gram’s iodine and allowed to stand for 1 minute. Tilted the slide gently and rinsed with distilled water. Decolorized with 95 percent ethyl alcohol and tilted the slide gently and applied alcohol drop by drop to 10 seconds. Immediately rinsed with distilled water gently flooded with safranin to counter stain and allowed to stand for 1 minute. Tilted the slide gently and rinsed with distilled water, dried the slide. Viewed the smear under 100X microscope.

Endophytic bacteria isolated from leaves of Tamarindus indica from Jawaharlal Nehru Krishi Viswa Vidyalaya JN- (J.N.K.V.V), Tropical Forest Research TF-(T.F.R.I.), State Forest Research Institute SF-(S.F.R.I) Jabalpur, showed gram positive reaction and were found to be rods.

The endophytic bacteria isolated from Tamarindus indica had shown positive reaction to Catalase test, Coagulase test, Voges-Proskauer (VP), orthonitrophenyl β-galactoside (ONPG), Urease, Arginine utilization and Sugar fermentation tests were done. Jawaharlal Nehru Krishi Viswa Vidyalaya JN- (J.N.K.V.V), Tropical Forest Research TF-(T.F.R.I.), State Forest Research Institute SF- (S.F.R.I) Jabalpur.

The endophytic bacteria isolated from Tamarindus indica had shown positive reaction to catalase, Vogasproskaur’s (VP) Orthro-Nitrophenyl-β-galactoside (ONPG), urease and negative reaction to coagulase, arginine utilization test.