The subject Plant Pathology is an important branch under the discipline Agriculture. This branch deals with the detection and diagnosis of agriculturally important diseases, symptoms, their causal agents, favourable environment and management aspects. Being an applied science and detailed study of plant diseases, laboratory work under this subject is indispensable.Keeping this view, ICAR has framed a syllabus for practical classes of each courses of Plant Pathology for undergraduate students. Therefore, we have tried to prepare a laboratory manual for the course of “Diseases of Field and Horticultural crops and Their Management” for benefits of the students. In this manual, we have tried to arrange topics and contents in such a manner that students will get an easy understanding of the principles and methods of the experiment in advance. The content of this manual is framed according to the new syllabus of the course- “Diseases of Field and Horticultural crops and Their Management” as mentioned in ICAR Sixth Dean Committee.